steponeplus real time pcr system (Thermo Fisher)
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Thermo Fisher
steponeplus real time pcr system
Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus+real+time+pcr+system/nct03277170-14-53-50
Average 90 stars, based on 1 article reviews
Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus+real+time+pcr+system/nct03277170-14-53-50
Average 90 stars, based on 1 article reviews
steponeplus real time pcr system - by Bioz Stars,
2026-09
90/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Adventitial Fibrosis and Fibroblast Mechanosensitivity Are Shaped by Sex and Hormonal Status in Pulmonary Arterial Hypertension Article Snippet: .. Quantitative PCR (qPCR) was performed with the KAPA SYBR FAST universal qPCR kit (#07959362001, Kapa Biosystems, Wilmington, MA, USA) on a Article Title: Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells Article Snippet: After cell harvest and PEG precipitation of the supernatant, AAV vectors were purified using iodixanol (OptiPrep, STEMCELL Technologies #07820) gradient ultracentrifugation. .. Vector titers were quantified by qPCR following DNase I (NEB #B0303S) treatment and Proteinase K (Qiagen #1114886) digestion, using primers specific to the viral genome. qPCR reactions were conducted with SsoFast EvaGreen Supermix (Bio-Rad #1725201) on a Article Title: Disturbed flow induces reprogramming of endothelial cells to immune-like and foam cells under hypercholesterolaemia during atherogenesis Article Snippet: Total RNA was isolated from cells lysed in TRIzol TM Reagent (Thermo Fisher Scientific, Waltham, MA, #15596018) and purified using the Direct-zol RNA Miniprep Kit (Zymo Research, Irvine, CA, #R2052), following the manufacturer’s protocol. .. Reverse transcription was carried out using PrimeScript RT Master Mix (Takara Bio, San Jose, CA, #RR036B) to generate cDNA. qPCR was then performed with PerfeCTa SYBR Green FastMix (QuantaBio, Beverly, MA, #95073–05K) on the Article Title: A calcium-sensing MCTP1/FYN/MEF2C circuit drives therapy-induced neuroendocrine prostate cancer Article Snippet: .. Total RNA was extracted using the RNeasy Midi Kit (Qiagen, Cat# 75142) according to the manufacturer’s instructions. cDNA was synthesized using a reverse transcription kit (Applied Biosystems, Cat# 4374966), and qPCR was conducted with SYBR Green Master Mix (Applied Biosystems, Cat# 4309155) on a Article Title: Compositions of autophagy modulating agents and uses thereof Article Snippet: Reverse transcription was performed using the SuperScript Ill first strand synthesis system for RT-PCR (Invitrogen, 18080-051) according to the manufacturer's protocol. .. Quantitative PCR was performed using SYBR Green PCR master mix (Applied Biosystems, 4309155) and Article Title: Protocol for differentiating patient-derived iPSCs into photoreceptor-like cells Article Snippet: ABI PRISM 3130xl Genetic Analyzer , Applied Biosystems , Cat# 3130xl. .. Article Title: Niosome-Encapsulated Sodium Butyrate Enhances Blood-Brain Barrier Integrity and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke. Article Snippet: Subsequently, 1 μg of total RNA was transcribed into complementary cDNA based on the manufacturer’s protocol of PrimeScript 1st strand cDNA synthesis kit (Cat# 6110A, Takara Bio Inc., Otsu, Japan). .. The cDNA amplification was conducted by a Article Title: Pragmatic RCT of High-dose Oral Montelukast for Moderate and Severe Pediatric Acute Asthma Exacerbations Article Snippet: .. PCR detection of respiratory viruses: Testing for influenza A, B, and C; RSV; adenovirus; enterovirus; human metapneumovirus; human rhinovirus; parainfluenza 1-4; coronavirus 229E, NL63, OC43, and HKU1; and human bocavirus can be performed according to established protocols using optimized target-specific primers and FAM/BHQ1-congugated hydrolysis probes, AgPath-ID One Step RT-PCR chemistry (Applied Biosystems), and Reverse Transcription:Article Title: Disturbed flow induces reprogramming of endothelial cells to immune-like and foam cells under hypercholesterolaemia during atherogenesis Article Snippet: Total RNA was isolated from cells lysed in TRIzol TM Reagent (Thermo Fisher Scientific, Waltham, MA, #15596018) and purified using the Direct-zol RNA Miniprep Kit (Zymo Research, Irvine, CA, #R2052), following the manufacturer’s protocol. .. Reverse transcription was carried out using PrimeScript RT Master Mix (Takara Bio, San Jose, CA, #RR036B) to generate cDNA. qPCR was then performed with PerfeCTa SYBR Green FastMix (QuantaBio, Beverly, MA, #95073–05K) on the Article Title: A calcium-sensing MCTP1/FYN/MEF2C circuit drives therapy-induced neuroendocrine prostate cancer Article Snippet: .. Total RNA was extracted using the RNeasy Midi Kit (Qiagen, Cat# 75142) according to the manufacturer’s instructions. cDNA was synthesized using a reverse transcription kit (Applied Biosystems, Cat# 4374966), and qPCR was conducted with SYBR Green Master Mix (Applied Biosystems, Cat# 4309155) on a SYBR Green Assay:Article Title: Disturbed flow induces reprogramming of endothelial cells to immune-like and foam cells under hypercholesterolaemia during atherogenesis Article Snippet: Total RNA was isolated from cells lysed in TRIzol TM Reagent (Thermo Fisher Scientific, Waltham, MA, #15596018) and purified using the Direct-zol RNA Miniprep Kit (Zymo Research, Irvine, CA, #R2052), following the manufacturer’s protocol. .. Reverse transcription was carried out using PrimeScript RT Master Mix (Takara Bio, San Jose, CA, #RR036B) to generate cDNA. qPCR was then performed with PerfeCTa SYBR Green FastMix (QuantaBio, Beverly, MA, #95073–05K) on the Article Title: A calcium-sensing MCTP1/FYN/MEF2C circuit drives therapy-induced neuroendocrine prostate cancer Article Snippet: .. Total RNA was extracted using the RNeasy Midi Kit (Qiagen, Cat# 75142) according to the manufacturer’s instructions. cDNA was synthesized using a reverse transcription kit (Applied Biosystems, Cat# 4374966), and qPCR was conducted with SYBR Green Master Mix (Applied Biosystems, Cat# 4309155) on a Article Title: Compositions of autophagy modulating agents and uses thereof Article Snippet: Reverse transcription was performed using the SuperScript Ill first strand synthesis system for RT-PCR (Invitrogen, 18080-051) according to the manufacturer's protocol. .. Quantitative PCR was performed using SYBR Green PCR master mix (Applied Biosystems, 4309155) and Article Title: Niosome-Encapsulated Sodium Butyrate Enhances Blood-Brain Barrier Integrity and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke. Article Snippet: Subsequently, 1 μg of total RNA was transcribed into complementary cDNA based on the manufacturer’s protocol of PrimeScript 1st strand cDNA synthesis kit (Cat# 6110A, Takara Bio Inc., Otsu, Japan). .. The cDNA amplification was conducted by a Synthesized:Article Title: A calcium-sensing MCTP1/FYN/MEF2C circuit drives therapy-induced neuroendocrine prostate cancer Article Snippet: .. Total RNA was extracted using the RNeasy Midi Kit (Qiagen, Cat# 75142) according to the manufacturer’s instructions. cDNA was synthesized using a reverse transcription kit (Applied Biosystems, Cat# 4374966), and qPCR was conducted with SYBR Green Master Mix (Applied Biosystems, Cat# 4309155) on a Polymerase Chain Reaction:Article Title: Compositions of autophagy modulating agents and uses thereof Article Snippet: Reverse transcription was performed using the SuperScript Ill first strand synthesis system for RT-PCR (Invitrogen, 18080-051) according to the manufacturer's protocol. .. Quantitative PCR was performed using SYBR Green PCR master mix (Applied Biosystems, 4309155) and Article Title: Pragmatic RCT of High-dose Oral Montelukast for Moderate and Severe Pediatric Acute Asthma Exacerbations Article Snippet: .. PCR detection of respiratory viruses: Testing for influenza A, B, and C; RSV; adenovirus; enterovirus; human metapneumovirus; human rhinovirus; parainfluenza 1-4; coronavirus 229E, NL63, OC43, and HKU1; and human bocavirus can be performed according to established protocols using optimized target-specific primers and FAM/BHQ1-congugated hydrolysis probes, AgPath-ID One Step RT-PCR chemistry (Applied Biosystems), and Amplification:Article Title: Niosome-Encapsulated Sodium Butyrate Enhances Blood-Brain Barrier Integrity and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke. Article Snippet: Subsequently, 1 μg of total RNA was transcribed into complementary cDNA based on the manufacturer’s protocol of PrimeScript 1st strand cDNA synthesis kit (Cat# 6110A, Takara Bio Inc., Otsu, Japan). .. The cDNA amplification was conducted by a One Step RT-PCR:Article Title: Pragmatic RCT of High-dose Oral Montelukast for Moderate and Severe Pediatric Acute Asthma Exacerbations Article Snippet: .. PCR detection of respiratory viruses: Testing for influenza A, B, and C; RSV; adenovirus; enterovirus; human metapneumovirus; human rhinovirus; parainfluenza 1-4; coronavirus 229E, NL63, OC43, and HKU1; and human bocavirus can be performed according to established protocols using optimized target-specific primers and FAM/BHQ1-congugated hydrolysis probes, AgPath-ID One Step RT-PCR chemistry (Applied Biosystems), and |